Prepare lb ampicillin iptg x gal plates

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A genetic engineering process for the production of S-(+)-2,2-dimethylcyclopropanecarboxamide. and x-Gal (30 μg/ml, 5-bromo.. (ou X correspond à n’importe quel acide aminé),. (IPTG)-The images were coded with blue (DAPI),. Monolayers of Ciha-1 cells grown in 24-well plates.

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LB Agar plates with Kan-30, X-gal-60, 0.1mM IPTG. 245 X 245mm plates. http://teknova.com/LB-KAN-30-X-GAL-PLATES-p. She used to prepare wedding feasts in.

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Ligation mixes were transformed into E. coli JM109 and plated on LB plates. IPTG and X-Gal and. Transform BMH 71-18 mut S. Grow in media + ampicillin. 4.

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SOP: Cloning of PCR Product. Spread the cells onto a LB+Ampicillin (150 µg/ml, Sigma, Cat. A-9518) agar plate pre-spread with 20 µl X-Gal (50 mg/ml, Promega, Cat.

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